weri rb1 cell line  (ATCC)

Journal: Cancers

Article Title: Gastric Inhibitory Polypeptide Receptor (GIPR) Overexpression Reduces the Tumorigenic Potential of Retinoblastoma Cells

doi: 10.3390/cancers16091656

Figure Lengend Snippet: Verification of GIPR upregulation after lentiviral TFF1 overexpression in two retinoblastoma cell lines. ( a ) Verification of TFF1 overexpression in Weri and Y79 RB cells via Real-Time PCR. ( b ) After lentiviral TFF1 overexpression in two RB cell lines, GIPR is significantly upregulated at the mRNA level, as revealed by Real-Time PCR. ( c ) In TFF1-overexpressing Weri RB cells, GIPR is likewise upregulated at the protein level, as revealed by a Western blot analysis. CTRL = cells transduced with control vector; TFF1 OE = TFF1 overexpression. Values represent the means ± SEM; significances are calculated by an unpaired Student’s t -test. ns: not significant; * p < 0.05; and *** p < 0.001.

Article Snippet: The RB cell lines WERI-Rb1 (Weri [ ] and Y79 [ ], initially acquired from the Leibniz Institute DSMZ (German Collection of Microorganisms and Cell Cultures), were generously supplied by Dr. H. Stephan.

Techniques: Over Expression, Real-time Polymerase Chain Reaction, Western Blot, Transduction, Control, Plasmid Preparation

Journal: Cancers

Article Title: Gastric Inhibitory Polypeptide Receptor (GIPR) Overexpression Reduces the Tumorigenic Potential of Retinoblastoma Cells

doi: 10.3390/cancers16091656

Figure Lengend Snippet: Verification of GIPR overexpression in retinoblastoma cell lines. ( a ) Verification of GIPR overexpression (OE) in the retinoblastoma (RB) cell lines Weri and Y79 on mRNA level via Real-Time PCR. ( b ) Verification of GIPR overexpression at the protein level via Western blot analysis in the RB cell lines Weri and Y79. ( c ) Immunofluorescent stains against GIPR (red fluorescence) with DAPI (blue) counterstaining after GIPR overexpression in Weri RB cells. Scale bar: 50 µm (applies to all pictures in ( c ). CTRL = cells transduced with control vector; GIPR OE = GIPR overexpression. Values represent the means ± SEM; significances are calculated by an unpaired Student’s t -test. * p < 0.05; ** p < 0.01.

Article Snippet: The RB cell lines WERI-Rb1 (Weri [ ] and Y79 [ ], initially acquired from the Leibniz Institute DSMZ (German Collection of Microorganisms and Cell Cultures), were generously supplied by Dr. H. Stephan.

Techniques: Over Expression, Real-time Polymerase Chain Reaction, Western Blot, Fluorescence, Transduction, Control, Plasmid Preparation

Journal: Cancers

Article Title: Gastric Inhibitory Polypeptide Receptor (GIPR) Overexpression Reduces the Tumorigenic Potential of Retinoblastoma Cells

doi: 10.3390/cancers16091656

Figure Lengend Snippet: In vitro effects of GIPR overexpression in retinoblastoma cell lines. ( a ) Cell viability was significantly decreased following GIPR overexpression (GIPR OE; blue bars) in the retinoblastoma (RB) cell lines Weri and Y79, as revealed by WST-1 assays. ( b , c ) Growth kinetics of Weri ( b ) and Y79 ( c ) RB cells were significantly decreased after GIPR overexpression. ( d ) Proliferation of Weri and Y79 cells was significantly decreased after GIPR overexpression, as revealed by the quantification of BrdU stains. ( e ) The significant increase in apoptosis after GIPR overexpression in Weri and Y79 cells was caspase-3-dependent, as revealed by the quantification of immunocytochemical stains against cleaved caspase-3. ( f ) Immunocytochemical stains against cleaved caspase-3 in Weri control cells (CTRL) and GIPR-overexpressing (GIPR OE) Weri cells. Arrowheads indicate cleaved caspase-3-positive (cleaved caspase-3 + ) cells. Scale bar 50 µm. CTRL = cells transduced with control vector. Values represent the means ± SEM; significances were calculated by an unpaired Student’s t -test. ns = p > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001; and **** p < 0.0001.

Article Snippet: The RB cell lines WERI-Rb1 (Weri [ ] and Y79 [ ], initially acquired from the Leibniz Institute DSMZ (German Collection of Microorganisms and Cell Cultures), were generously supplied by Dr. H. Stephan.

Techniques: In Vitro, Over Expression, Control, Transduction, Plasmid Preparation

Journal: Cancers

Article Title: Gastric Inhibitory Polypeptide Receptor (GIPR) Overexpression Reduces the Tumorigenic Potential of Retinoblastoma Cells

doi: 10.3390/cancers16091656

Figure Lengend Snippet: Effects of administration of a GIPR inhibitor and recombinant TFF1 on GIPR-overexpressing RB cells. ( a , d ) Cell viability was significantly decreased following GIPR overexpression (GIPR OE; blue bars) in the retinoblastoma (RB) cell lines Weri ( a ) and Y79 ( d ), as revealed by WST-1 assays after 24 h. After the administration of the GIPR inhibitor MK0893, the effect was reversed. The addition (+) of recombinant TFF1 (rTFF1) or a combination of MK0893 and rTFF1 did not lead to changes in cell viability compared to untreated GIPR-overexpressing cells (−). ( b , e ) Cell proliferation in Weri ( b ) as well as in Y79 ( c ) cells was decreased after GIPR overexpression, as revealed by the quantification of BrdU stains. Following the administration of MK0893, the effect was reversed, and the proliferation levels exceeded those of the control cells, transduced with a control vector (CTRL). The addition of rTFF1 did not lead to changes in proliferation compared to the untreated GIPR-overexpressing cells. ( c , f ) Changes in the cell death levels after GIPR overexpression were revealed by the counting of trypan blue-positive cells. GIPR overexpression resulted in an increased apoptosis level of Weri ( c ) and Y79 ( f ) cells. Following the administration of MK0893, the cell death levels dropped significantly in Y79 ( f ) but not in the Weri cell line. The addition of rTFF1 did not lead to significant changes compared to the cell death levels of the untreated controls. The legends in a and d also apply to all the other graphs. Values represent the means ± SEM; significances are calculated by an unpaired Student’s t -test. ns = p > 0.05; * p < 0.05; ** p < 0.01; and *** p < 0.001.

Article Snippet: The RB cell lines WERI-Rb1 (Weri [ ] and Y79 [ ], initially acquired from the Leibniz Institute DSMZ (German Collection of Microorganisms and Cell Cultures), were generously supplied by Dr. H. Stephan.

Techniques: Recombinant, Over Expression, Control, Transduction, Plasmid Preparation

Journal: Cancers

Article Title: Gastric Inhibitory Polypeptide Receptor (GIPR) Overexpression Reduces the Tumorigenic Potential of Retinoblastoma Cells

doi: 10.3390/cancers16091656

Figure Lengend Snippet: Endogenous GIPR and miR-542-5p expression levels in RB cell lines and expression after miR-542-5p overexpression. ( a , b ) Compared to healthy human retina (hRet), the RB cell lines Weri and Y79 displayed increased GIPR (( a ); blue bars) and decreased miR-542-5p (( b ); green bars) mRNA expression levels, as revealed by Real-Time PCR. ( c , d ) After successful miR-542-5p overexpression (grey bars; ( c )), verified by Real-Time PCR, the RB cell lines displayed significantly decreased GIPR mRNA expression levels ( d ). CTRL = cells transduced with control vector. miR-542-5p OE = miR-542-5p overexpression. Values represent the means ± SEM; non-significant p -values are not shown. * p < 0.05; ** p < 0.01; *** p < 0.001; and **** p < 0.0001.

Article Snippet: The RB cell lines WERI-Rb1 (Weri [ ] and Y79 [ ], initially acquired from the Leibniz Institute DSMZ (German Collection of Microorganisms and Cell Cultures), were generously supplied by Dr. H. Stephan.

Techniques: Expressing, Over Expression, Real-time Polymerase Chain Reaction, Transduction, Control, Plasmid Preparation